3. curvatus, Lb. (4)  […], […] of multiple kombucha (tea fungus) samples či jeho čtivějšího a předžvýkaného excerpta Microbial diversity of kombucha, což je docela moderní poznatek, který interpretuje data z DNA sekvenceru. | Ep. 29/07/14 | Posted in kombucha by Benjamin Wolfe. The microbiologists in this paper were the first to use high-throughput (sometimes called next-generation) DNA sequencing techniques to analyze kombucha microbial diversity. Although Leuconostoc species were more dominant than Weissella and Lactobacillus species at the beginning of fermentation, Leuconostoc species increased again and Lactobacillus and Weissella decreased at the end of the fermentation. The reads that aligned with the Le. Ben. gasicomitatum, and Le. Great summary! Clustered regularly interspaced short palindromic repeats (CRISPR) elements were searched from assembled contigs longer than 10 kb by using the Web-based tool CRISPRFinder (18). The finding of lactic acid bacteria at high abundance suggests a potentially under-appreciated component of kombucha microbiology.

1). However, culture-based approaches have known limitations in terms of reproducibility and challenges in the culturability of some bacteria.

The concentration of purified genomic DNA was measured with an ELISA reader equipped with a Take3 multivolume plate (SynergyMx; BioTek, CA), and the DNA samples were concentrated to a minimum of 100 ng/μl using a speed vacuum centrifuge (Eyela CVE-2000; Japan). mesenteroides ATCC 8293T (NC_008531), Lactobacillus sakei 23K sakei (NC_007576), the partially assembled genome of Weissella paramesenteroides ATCC 33313, and Leuconostoc citreum KM20 (NC_010471). 3). Photo by Benjamin Wolfe. This wasn’t a huge surprise because previous studies had also observed dominance by Zygosaccharomyces in other samples.
Phylogenetic analysis based on the MCP sequences showed that contigs 02778, 27827, and 01645 were related to LAB phages (data not shown). Therefore, sequencing reads from the kimchi metagenome, which comprised a total of 560,907 clean reads, were assembled, resulting in a total of 27,835 contigs. The finding that not all komnucha samples are the same also suggests that each producer, whether a home or professional fermenter, can develop unique microbial communities.

1H-NMR spectra of the kimchi supernatants were associated with the presence of various metabolites, including carbohydrates, amino acids, and organic acids, as expected.
In contrast, kimchi fermented with microorganisms from kimchi garlic showed that strains of Weissella and Leuconostoc were dominant. Is it good or bad? Many bacteria are involved in the fermentation of kimchi, but LAB become dominant while the putrefactive bacteria are suppressed during salting of baechu cabbage and the fermentation.

The 16S rRNA gene copy number of each sample was calculated as described previously (41, 46). The population of Kombucha microbial community varies greatly.

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